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                  <text>Revista Oftalmología Clínica y Experimental</text>
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                <text>Regeneración corneal mediante tratamiento con vesículas extracelulares derivadas de células madre mesenquimales en un modelo murino de injuria alcalina&#13;
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                <text>Santa Cruz, Diego Mario </text>
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                <text>Cantero, María José </text>
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                <text>Fiore, Esteban </text>
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                <text>Pacienza, Natalia Alejandra </text>
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                <text>Objetivo&#13;
&#13;
En los últimos años, estudiamos las propiedades regenerativas, anti-inflamatorias e inmuno-moduladoras de las vesículas extracelulares (EVs) secretadas por células madre mesenquimales (MSCs). Debido a que los procesos que conllevan a la opacidad corneal se deben básicamente a una desregulación de mecanismos regenerativos e inflamatorios, en el presente trabajo, nos propusimos evaluar el potencial terapéutico de las EVs derivadas de MSCs en un modelo experimental de injuria corneal.&#13;
&#13;
Métodos&#13;
&#13;
Previamente, desarrollamos un sistema de aislamiento de vesículas extracelulares y posterior análisis de su potencial anti-inflamatorio (in vitro). Este sistema de producción y aislamiento de EVs comprendido por: cultivo controlado de MSCs (de cordón umbilical humano), recolección de medio condicionado y purificación de EVs mediante cromatografía de intercambio iónico. En este estudio, pusimos a punto un modelo murino de regeneración corneal mediante aplicación tópica de 10ul de NaOH (0,125 M) y posterior tratamiento con igual volumen de EVs (1x109 partículas/ml) por 5 días consecutivos. La acción terapéutica de las EVs se evaluó mediante tinción tisular (hematoxilina-eosina) y determinación de los niveles de expresión de citoquinas inflamatorias  por real-time RT-PCR.&#13;
&#13;
Resultados&#13;
&#13;
Tanto en la observación clínica, como en la tisular, se observa  que el NaOH indujo injuria moderada del tejido corneal con un claro restablecimiento del tejido luego del tratamiento con vesículas (vs vehículo). Respecto a las citoquinas evaluadas, estas aumentaron significativamente su expresión luego de la injuria química y descendieron fuertemente tras el tratamiento con EVs (n=5, p&lt;0.05).&#13;
&#13;
Conclusión&#13;
&#13;
De los resultados obtenidos, se desprende, que  logramos establecer un modelo sencillo de injuria del epitelio corneal en el cual testear diversas estrategias terapéuticas y, a su vez, demostramos  el enorme potencial regenerativo de las EVs en patologías oculares de orden inflamatorio.</text>
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                <text>Células madre mesenquimales estromales</text>
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                <text>Vesículas extracelulares</text>
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                <text>Consejo Argentino de Oftalmología</text>
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                <text>2025</text>
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                <text>Oftalmología Clínica y Experimental, vol. 18, nro. 2, jul. de 2025, p. 143-52</text>
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                <text>El óxido nítrico en el desprendimiento de retina y la proliferación vitreoretinal /  M. Valeria Cantó Soler, Juan E. Gallo, Ricardo A. Dodds y Angela M. Suburo.</text>
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                <text>2004.</text>
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                <text>vol. 77, nº 2 (oct-dic 2004), p. 261-278Título: Archivos de Oftalmología de Buenos Aires|773x|0066-6777</text>
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                <text>Desprendimiento de retina</text>
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                <text>Cantó Soler, M. Valeria.</text>
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                <text>Buenos Aires : Sociedad Argentina de Oftalmología,</text>
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                <text>ene.-mar.1986</text>
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                <text>Archivos de Oftalmología de Buenos Aires; Buenos Aires, ene.-mar.1986. ISSN: 0066-6777</text>
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                <text>Etica</text>
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                <text>Oftalmología</text>
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                <text>Código de ética</text>
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                <text>Capdehourat, Eduardo L.</text>
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                <text>Buenos Aires : Sociedad Argentina de Oftalmología,</text>
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                <text>Capeans Tomé, C.</text>
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                <text>Percepción visual. Psicofísica, mecanismos y modelos.</text>
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